Background: Tomography can be corrected by SCATTER correction (SC) to improve image quality.Siemens SPECT has several function of optional marked SC in the tomographic image reconstruction.The aim of this research is to distinguish the difference between the different function settings.Materials and Methods: "SC‐Tc‐99m‐NMG" energy peak of Brain model tomographic data were used.Dual lsotopes loaded in "Tomo Reconstruction" activity primary and secondary energy peak to analyze separately in 2 groups. Single lsotopes loaded in "Tomo Reconstruction" activity divided into SC and NSC to analyze separately in 2 groups. Dual lsotopes loaded in "AutoRecon" activity divided into SC and NSC to analyze separately in 2 groups. Limited by "AutoRecon" activity, we use Flash 3D (8, 8) gaussian 8.00 function to reconstruct and analyze the range of image to the largest extent.Results: With all 128 layers summed in axial section, the average radioactive counts of the 6 groups respectively were 10092.30±24.20, 4199.40±13.41, 10092.60±24.95, 10084.30±16.05, 8151.60±20.64 and 10238.80±20.64, and the maximum counts were 10328, 3621, 10370, 10338, 8546 and 10445. Except secondary energy peak data, others met the clinical requirements through visual assessment brain SPECT. There was no statistically significant differences between a, c, d groups after paired T test.Conclusions: SC could only be done with Flash 3D function in “AutoRecon” activity using Siemens syngo V60B.